ID: 1008 Quercus infectoria semi-purified fractions promoted BMP-2, Runx2 and osteopontin expression in human fetal osteoblastic cell line

Amira Raudhah Abdullah; Hermizi Hapidin; Hasmah Abdullah

doi:10.15419/bmrat.v4iS.289

Vol 4 No S (2017): Abstract Proceeding: International conference INNOVATIONS IN CANCER RESEARCH AND REGENERATIVE MEDICINE 2017 / S 85 Poster Abstract

ID: 1008 Quercus infectoria semi-purified fractions promoted BMP-2, Runx2 and osteopontin expression in human fetal osteoblastic cell line

Amira Raudhah Abdullah

Hermizi Hapidin

Hasmah Abdullah

More author's info

Amira Raudhah Abdullah
School of Health Science, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia

[email protected]

Hermizi Hapidin
School of Health Science, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia

Hasmah Abdullah
School of Health Science, Universiti Sains Malaysia, Kubang Kerian, Kelantan 16150, Malaysia

Submitted: Aug 31, 2017 | Published: Sep 5, 2017

Vol 4 No S (2017): Abstract Proceeding: International conference INNOVATIONS IN CANCER RESEARCH AND REGENERATIVE MEDICINE 2017 | DOI: 10.15419/bmrat.v4iS.289 | Page No.: S 85

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Abdullah, A., Hapidin, H., & Abdullah, H. (2017). ID: 1008 Quercus infectoria semi-purified fractions promoted BMP-2, Runx2 and osteopontin expression in human fetal osteoblastic cell line. Biomedical Research and Therapy, 4(S), S 85. https://doi.org/10.15419/bmrat.v4iS.289

Copyrights: Amira Raudhah Abdullah, Hermizi Hapidin, Hasmah Abdullah, 2017. License:

This work is licensed under a Creative Commons Attribution 4.0 International License.

Abstract

Quercus infectoria (QI) is traditional medicines in Asia which contain many active phytochemical compounds that have the potential to stimulate bone formation. However, the precise mechanism of the therapeutic effect of QI on osteoblast has not been elucidated. The present study was carried out to extract the active compound of QI through a series of purification in order to obtain semi-purified fractions of QI followed by examining its phytochemical profile through liquid chromatography mass spectrometry (LC-MS) as well as to delineate the molecular mechanism of QI semi-purified fraction-enhanced bone formation by investigating the protein expression of bone morphogenic protein-2 (BMP-2), Runx2 and osteopontin (OPN) in the hFOB 1.19 human fetal osteoblastic cell line. The semi-purified fractions of QI were produced in series of column chromatography and thin layer chromatography (TLC) technique. The obtained fractions were then tested in a series of bio-guided assay through MTT assay. Three most potent fractions with lowest EC50 value (Fraction A-10.85 µg/ml, Fraction B-12.00 µg/ml and Fraction C-11.60 µg/ml) were selected to be treated with hFOB1.19 cell followed by quantification of BMP-2, Runx2 and OPN protein expression at day 1, 3 and 7. Compound profiling through LC-MS analysis showed that the chosen fractions consist of main active component known as gallic acid that has been showed previously to promote bone formation. Results show that QI semi-purified fractions increased the activity of BMP-2, Runx2 and OPN at day 1 and day 3. Meanwhile as the expression of BMP-2 and Runx2 continue to increase at day 7, the expression of OPN shows a down regulation at day 7. Furthermore, the study showed that combination of Fraction A, B and C wit osteoporotic drug (pamidronate) further increase the expression of BMP-2 and Runx2 contrary to OPN expression. These finding demonstrated that semi-purified fraction of QI enhanced bone formation through expression of investigated bone-related marker.

Keywords: Bone Morphogenic Protein-2 (BMP-2) hFOB1.19 cell Osteopontin Quercus infectoria Stem cell Runx2