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Relationship of transgelin expression with clinicopathological characteristics and disease-free survival in HER2-positive breast cancer

Nguyen Thu Thuy 1
Nguyen Van Hung 2, *
Nguyen Van Chu 3, 4
Le Phong Thu 5
Le Trung Tho 1, 6
  1. Department of Pathology, Hanoi Medical University, Vietnam. 1, Ton That Tung Street, Dong Da District, Hanoi, Viet Nam
  2. Department of Pathology, Phenikaa University, Vietnam. Nguyen Trac Street, Ha Dong District, Hanoi, Viet Nam
  3. Department of Pathology, National Cancer Hospital, Hanoi, Vietnam. 43, Quan Su Street, Hoan Kiem District, Hanoi, Viet Nam
  4. Department of Clinical Pathology, Hanoi Medical University, Viet Nam. 1, Ton That Tung Street, Dong Da District, Hanoi, Viet Nam
  5. Department of Pathology, Thai Nguyen University of Medicine and Pharmacy, Vietnam, 284 Luong Ngoc Quyen Street, Thai Nguyen City, Thai Nguyen Province, Viet Nam
  6. Department of Pathology, National Lung Hospital, Ha Noi, Viet Nam, 463 Hoang Hoa Tham Street, Ba Dinh District, Hanoi, Viet Nam
Correspondence to: Nguyen Van Hung, Department of Pathology, Phenikaa University, Vietnam. Nguyen Trac Street, Ha Dong District, Hanoi, Viet Nam. Email: [email protected].
Volume & Issue: Vol. 12 No. 8 (2025) | Page No.: 7697-7707 | DOI: 10.15419/55xvxh21
Published: 2025-08-31

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This article is published with open access by BioMedPress. This article is distributed under the terms of the Creative Commons Attribution License (CC-BY 4.0) which permits any use, distribution, and reproduction in any medium, provided the original author(s) and the source are credited. 

Abstract

Background: Cancer-associated fibroblasts (CAFs) are essential for shaping the tumor microenvironment and influencing therapeutic responses in breast cancer. Thus, a better understanding of the expression of transgelin, a key marker of CAFs, may provide prognostic information. However, the prognostic significance of transgelin in human epidermal growth factor receptor 2 (HER2)-positive breast cancer remains unclear. Here, we investigated the relationship of transgelin expression with clinicopathological characteristics and survival outcomes in HER2-positive breast cancer.

Methods: Data were collected retrospectively from 111 HER2-positive breast cancer tissue samples. The density of tumor-infiltrating lymphocytes (TILs) was evaluated by hematoxylin and eosin staining. Immunohistochemical staining of transgelin and CD8 was conducted.

Results: Strong transgelin expression in CAFs was negatively associated with CD8 and TILs (p < 0.005). In the multivariate analysis, transgelin expression in CAFs (p = 0.023) and pathological stage (p = 0.029) were identified as independent prognostic factors for disease-free survival (DFS). The model combining transgelin expression in CAFs and pathological stage improved prediction (−2 log-likelihood = 74.062, Akaike information criterion [AIC] = 78.06) compared with pathological stage alone (−2 log-likelihood = 80.815, AIC = 82.81). Patients in the high-risk group had a shorter DFS (p < 0.0001).

Conclusions: Transgelin expression in CAFs appears to be a novel prognostic marker for HER2-positive breast cancer.

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