
DNA promoter methylation of cancer-associated genes in patients with head and neck squamous cell carcinoma
- Institute of Medicine, Peoples’ Friendship University of Russia (RUDN University), 117198 Moscow, Russia
- A. Tsyb Medical Radiological Research Center – branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation (A. Tsyb MRRC)
- P. Hertsen Moscow Oncology Research Institute - branch of the National Medical Research Radiological Center of the Ministry of Health of the Russian Federation (P. Hertsen MORI)
Abstract
Introduction: Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer in the world. Abnormal methylation can be one reason for this cancer. This study aimed to investigate the DNA promotor methylation status of cancer-associated genes (ATM, APC, CDO1, RB1, TP53, and WIF1) in patients with HNSCC.
Methods: Bisulfite conversion and methylation-sensitive high-resolution melting were used to analyze the DNA methylation levels in normal and tumor tissues in 44 patients.
Results: Significant differences in DNA methylation were observed between tumor and normal tissues for CDO1 and WIF1 genes in all subjects and subgroups (p < 0.05). In the T3 subgroup, a significant correlation was found between CDO1 gene methylation and age in normal tissue. The same correlation was detected for the WIF1 gene methylation in tumor tissue samples in the subgroup with T3 and normal tissue samples in the subgroup with T4 (p < 0.05). In all genes, no significant differences were found between the patient subgroups (T2, T3, T4 stage, primary/recurrent lesion, non-keratinizing/keratinizing SCC, age before/after 50, and smokers/non-smokers).
Conclusion: Changes in the expressions of CDO1 and WIF1 genes can affect the mechanisms of the occurrence and development of HNSCC. Methylation in the ATM, APC, RB1, and TP53 genes is not specific to HNSCC.
Introduction
Epigenetic gene regulation changes (such as DNA methylation, histone modification, chromatin remodeling, and non-coding RNA) and DNA mutation are the basis of the molecular mechanisms of cancerogenesis and tumorigenesis. Therefore, studying epigenetic markers for diagnosis, prognosis, and cancer prevention is particularly interesting. Detection of DNA methylation levels has recently been used as biomarkers for early diagnosis and prognosis to identify target genes for drug therapy.
The hypomethylation of single CpG dinucleotides has been one of the first changes found in tumor cells1. A large part of the investigation has been carried out during DNA methylation in the promoter region of various genes2, 3. There are changes in the methylation levels of general and tissue-specific cell genes in the ontogenesis and post-natal periods.
The non-mutagenic etiology of cancer, such as disturbance of DNA methylation, is influenced by age, diet, transplacental, environmental, and occupational exposure. Moreover, its dual ability to be reversible and transgenerational demands a specific approach to interpreting results. Age-related DNA methylation is associated with different diseases4 and sex hormone status in some cancer types5. There is a significant gap in understanding the association between the age-expected DNA methylation level and cancer6. In particular, no data exist regarding pre- and postmenopausal differences in DNA methylation levels and their effects on cancer development.
The most intensive processes are in the genome of active proliferating cells, such as epithelial cells. Therefore, epithelial cells in various cancers are convenient models for studying epigenetic malformation. Head and neck squamous cell carcinoma (HNSCC) is an example of oncopathology with epithelial tissue damage.
HNSCC corresponds to the majority of cases (~90%) of head and neck cancers (HNCs)7. HNCs are rated 6–7 in global prevalence among cancers, with 700,000 newly registered cases and 470,000 deaths a year8. The therapeutic options for HNCs include surgical treatment, radiotherapy, and chemotherapy. The management tactics should be personalized by a multidisciplinary board comprising an oncological surgeon, a chemotherapist, a radiotherapist, a psychologist, a rehabilitation specialist, and a dentist9, 10.
Despite the advanced diagnostics, cancer screening, and HPV vaccination programs, 60–70% of the cases are diagnosed as late as during stage III–IV, associated with low life expectancy and high risks of recurrence11. Therefore, finding new potential targets for therapy is crucial.
The main risk factor for HNSCC is smoking. In some publications, it has been shown that smokers have a 10-fold higher risk of developing the disease than non-smokers. Smoking, in combination with frequent alcohol consumption, increases the risk more than 35-fold12, 13, 14. Other risk factors include ultraviolet and ionizing radiation, various toxic compounds, a weakened immune system, a diet low in vitamins A and B, age over 40, male, and some viral infections15.
DNA methylation also varies due to several factors, including age and disease status. Silenced genes are often methylated, while active genes remain largely unmethylated16, 17. DNA methylation is the most studied epigenetic mechanism in HNSCC. It allows us to observe changes in methylation patterns in a whole genome and individual genes that can be used to identify new disease biomarkers18. Various biomaterials can be used for studies, including liquid biopsy samples, which provide a noninvasive alternative for early cancer detection. In particular, Zhou C. studied 27 aberrantly methylated genes with altered expression and showed that FAM135B methylation is a favorable independent prognostic marker for overall survival in patients with HNSCC19.
We previously investigated the DNA methylation status of some tumor-associated genes (, and ) in patients with HNSCC20. We observed significant differences in DNA methylation levels between tumor and normal tissues in the and genes in all patient groups and subgroups (larynx and other cancers, SCC keratinizing and non-keratinizing, primary and recurrent tumor, and smokers and non-smokers)21. The methylation level in the gene in tumor tissue was significantly increased in the T and T stage subgroups over the T stage22.
For further research, we investigated another group of tumors and normal tissue from patients with HNSCC. We chose other cancer-associated genes: and . We also used these earlier studied genes ( and ), as they had shown notable results in other patients (
This study aimed to investigate the DNA promotor methylation status of cancer-associated genes (and) in patients with HNSCC.
Characteristics of studied genes
Symbol |
Gene name |
Location |
Exon count |
Gene ID |
Transcripts |
MIM |
Gene type |
Gene function |
References |
---|---|---|---|---|---|---|---|---|---|
APC GS; DP2; DP3; BTPS2; DESMD; DP2.5; PPP1R46 |
Adenomatous Polyposis Coli |
5q22.2 |
20 |
5624 |
NM_000038.6 |
611731 |
protein-coding |
tumor suppressor |
37; 39 |
ATM AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1 |
Ataxia-telangiectasia-mutated |
11q22.3 |
67 |
472 |
NM_000051.4 |
607585 |
protein-coding |
cell cycle regulation. |
42-44 |
CDO1 (CDO-I) |
Cysteine dioxygenase type 1 |
5q22.3 |
9 |
1036 |
NM_001323565.2 |
603943 |
protein-coding |
tumor suppressor |
26-28 |
TP53 (P53; BCC7; LFS1; BMFS5; TRP53) |
Tumor Protein P53 |
17p13.1 |
11 |
7157 |
NM_000546.6. |
191170 |
protein-coding |
tumor suppressor |
45-50 |
RB1 (RB; pRb; OSRC; pp110; p105-Rb; PPP1R130; p110-RB1) |
Retinoblastoma 1 |
13q14.2 |
27 |
5925 |
NM_000321.3... |
614041 |
protein-coding |
cell cycle regulator, tumor suppressor |
51-53 |
WIF1 (WIF-1) |
WNT inhibitory factor 1 |
12q14.3 |
10 |
11197 |
NM_007191.5 |
605186 |
protein-coding |
tumor suppressor |
19; 30-36 |
Characteristics of patient
Patient ID |
Tumor origin |
ICD-10 |
TNM classification |
Type of lesion |
Histological glade |
Gender |
Age |
Smoker |
---|---|---|---|---|---|---|---|---|
1 |
Tongue n=9 |
C02.0 |
T1N0M0 |
Prim |
SCC non-keratinizing |
М |
50 |
y |
2 |
C02.1 |
T2N0M0 |
Prim |
SCC non-keratinizing |
F |
59 |
n | |
3 |
C02.1 |
T3N0M0 |
Rec |
SCC keratinizing |
F |
66 |
y | |
4 |
C02.1 |
T3N0M0 |
Rec |
SCC non-keratinizing |
F |
47 |
n | |
5 |
C02.1 |
T3N0M0 |
Prim |
SCC keratinizing |
М |
63 |
n | |
6 |
C02.1 |
T3N0M0 |
Prim |
SCC keratinizing |
М |
40 |
n | |
7 |
C02.1 |
T3N0M0 |
Prim |
SCC keratinizing |
M |
46 |
n | |
8 |
C02.1 |
T3N1M0 |
Prim |
SCC non-keratinizing |
М |
52 |
n | |
9 |
C02.1 |
T4N2M0 |
Prim |
SCC keratinizing |
М |
36 |
n | |
10 |
Oral cavity n=6 |
C03.0 |
T2N0M0 |
Prim |
SCC non-keratinizing |
F |
59 |
n |
11 |
C03.0 |
T3N0M0 |
Prim |
SCC keratinizing |
M |
69 |
y | |
12 |
C03.0 |
T4N0M0 |
Prim |
SCC keratinizing |
М |
37 |
y | |
13 |
C03.1 |
T4N0M0 |
Prim |
SCC non-keratinizing |
М |
30 |
n | |
14 |
C03.1 |
T4N0M0 |
Prim |
SCC non-keratinizing |
F |
63 |
n | |
15 |
C03.1 |
T4N0M0 |
Prim |
SCC non-keratinizing |
F |
48 |
n | |
16 |
Floor of mouth n=3 |
C04.1 |
T2N0M0 |
Prim |
SCC keratinizing |
F |
67 |
y |
17 |
C04.1 |
T3N1M0 |
Prim |
SCC non-keratinizing |
F |
66 |
y | |
18 |
C04.1 |
T4N0M0 |
Prim |
SCC keratinizing |
F |
64 |
y | |
19 |
Maxillary sinus n=6 |
C31.0 |
T3N0M0 |
Prim |
SCC non-keratinizing |
M |
58 |
y |
20 |
C31.1 |
T3N0M0 |
Prim |
SCC non-keratinizing |
M |
55 |
y | |
21 |
C31.8 |
T3N0M0 |
Rec |
SCC non-keratinizing |
M |
64 |
y | |
22 |
C31.0 |
T4N0M0 |
Rec |
SCC non-keratinizing |
M |
41 |
n | |
23 |
С31.8 |
T4aN0M0 |
Prim |
SCC non-keratinizing |
M |
61 |
n | |
24 |
C31.0 |
T4aN0M0 |
Prim |
SCC non-keratinizing |
M |
67 |
n | |
25 |
Larynx n=20 |
C32.1 |
T1N2M0 |
Prim |
SCC non-keratinizing |
М |
48 |
n |
26 |
C32.0 |
T2N0M0 |
Prim |
SCC non-keratinizing |
F |
55 |
n | |
27 |
С32.8 |
T2N0M0 |
Prim |
SCC non-keratinizing |
M |
70 |
n | |
28 |
С32.0 |
T2N0M0 |
Prim |
SCC non-keratinizing |
M |
69 |
y | |
29 |
C32.0 |
T2N0M0 |
Prim |
SCC non-keratinizing |
М |
58 |
y | |
30 |
C32.0 |
T3N0M0 |
Prim |
SCC keratinizing |
M |
64 |
y | |
31 |
C32.0 |
T3N0M0 |
Prim |
SCC non-keratinizing |
M |
62 |
n | |
32 |
C32.0 |
T3N0M0 |
Prim |
SCC keratinizing |
М |
62 |
n | |
33 |
C32.8 |
T3N0M0 |
Rec |
SCC non-keratinizing |
М |
64 |
y | |
34 |
C32.8 |
T3N0M0 |
Prim |
SCC non-keratinizing |
М |
69 |
y | |
35 |
C32.8 |
T3N0M0 |
Prim |
SCC non-keratinizing |
М |
58 |
y | |
36 |
C32.9 |
T3N0M0 |
Prim |
SCC keratinizing |
M |
61 |
n | |
37 |
C32.8 |
T3N1M0 |
Prim |
SCC non-keratinizing |
M |
49 |
y | |
38 |
C32.8 |
T3N0M0 |
Rec |
SCC non-keratinizing |
М |
69 |
n | |
39 |
C32.0 |
T4N2M0 |
Prim |
SCC non-keratinizing |
М |
64 |
n | |
40 |
C32.0 |
T4N0M0 |
Prim |
SCC keratinizing |
М |
71 |
y | |
41 |
C32.8 |
T4aN0M0 |
Prim |
SCC keratinizing |
M |
72 |
y | |
42 |
C32.8 |
T4aN0M0 |
Prim |
SCC non-keratinizing |
M |
58 |
n | |
43 |
С32.8 |
T4aN2aM0 |
Prim |
SCC non-keratinizing |
M |
70 |
y | |
44 |
C32.8 |
T4aN2bM0 |
Rec |
SCC non-keratinizing |
F |
81 |
n |
Primer sequences used for Methylation-Specific PCR (MSP)
Genes |
Forward primer sequence (5’ → 3’) |
Reverse primer sequence (5’ → 3’) |
Product size (bp*) |
---|---|---|---|
ATM |
GTTGGTTATTGGTGGATATGG |
TAATTCCAAAACCCAAACTCTTAAC |
696 |
APC |
GTTGGTTATTGGTGGATATGG |
AACCTACAAAACCAAAAACCAACTA |
600 |
CDO1 |
GGGAGGATGA ATTTTATAGATTTG |
TAAACTTCCATA ATAACCTACACCTC |
396 |
RB1 |
GATAGGGATGAGGTTTATAGTTATTTATTA |
AAAATCCTATCACCATTCTACAAAC |
770 |
TP53 |
GGATTATTTGTTTTTATTTGTTATGG |
CAAAACTCCACTCCTCTACCTAAAC |
495 |
WIF1 |
GAGTGATGTT TTAGGGGT |
CCTCAACCA AAACTATTCC |
464 |
Average level of promotor genes methylation in tumor and normal tissue in all patient and their subgroups. T-tumor, N-normal
Patients (n) Genes |
Sample |
All (n=44) |
TNM ** |
Type of lesion |
SCC |
Age |
Smokers | ||||||
T2 (n=7) |
T3 (n=20) |
T4 (n=15) |
Prim (n=37) |
Rec (n=7) |
non-keratinizing (n=30) |
keratinizing (n=14) |
before 50 years (n=10) |
(n=34) after 50 years |
Yes (n=20) |
No (n=24) | |||
APC |
T |
0.36±0.17 (0.01÷0.54) |
0.31±0.14 (0.05÷0.47) |
0.32±0.18 (0.01÷0.54) |
0.31±0.16 (0.01÷0.52) |
0.30±0.18 (0.01÷0.54) |
0.36±0.16 (0.01÷0.47) |
0.36±0.14 (0.01÷0.54) |
0.24±0.20 (0.01÷0.52) |
0.32±0.17 (0.01÷0.50) |
0.31±0.17 (0.01÷0.54) |
0.31±0.16 (0.01÷0.50) |
0.31±0.20 (0.01÷0.54) |
N |
0.28±0.18 (0.01÷0.56) |
0.22±0.18 (0.07÷0.48) |
0.31±0.18 (0.04÷0.56) |
0.24±0.18 (0.01÷0.56) |
0.27±0.19 (0.01÷0.56) |
0.34±0.14 (0.01÷0.46) |
0.32±0.17 (0.01÷0.56) |
0.21±0.19 (0.01÷0.56) |
0.27±0.18 (0.04÷0.50) |
0.28±0.19 (0.01÷0.56) |
0.27±0.17 (0.01÷0.50) |
0.29±0.20 (0.01÷0.56) | |
ATM |
T |
0.35±0.18 (0.01÷0.54) |
0.31±0.20 (0.04÷0.54) |
0.34±0.19 (0.01÷0.54) |
0.37±0.18 (0.06÷0.54) |
0.34±0.19 (0.01÷0.54) |
0.38±0.19 (0.01÷0.53) |
0.39±0.18 (0.01÷0.54) |
0.28±0.18 (0.01÷0.53) |
0.30±0.23 (0.01÷0.53) |
0.37±0.17 (0.04÷0.54) |
0.33±0.19 (0.01÷0.54) |
0.37±0.18 (0.04÷0.54) |
N |
0.30±0.19 (0.01÷0.57) |
0.27±0.22 (0.06÷0.57) |
0.30±0.19 (0.01÷0.56) |
0.29±0.17 (0.07÷0.49) |
0.31±0.19 (0.02÷0.57) |
0.27±0.21 (0.01÷0.49) |
0.36±0.18 (0.01÷0.57) |
0.20±0.18 (0.01÷0.55) |
0.29±0.19 (0.02÷0.50) |
0.30±0.19 (0.01÷0.57) |
0.30±0.18 (0.02÷0.57) |
0.30±0.20 (0.01÷0.56) | |
CDO1 |
T |
0.41±0.16* (0.18÷0.76) |
0.30±0.11 (0.18÷0.48) |
0.44±0.16* (0.18÷0.76) |
0.42±0.16* (0.18÷0.71) |
0.38±0.15* (0.18÷0.71) |
0.51±0.18* (0.26÷0.76) |
0.43±0.15* (0.18÷0.71) |
0.36±0.16* (0.18÷0.66) |
0.38±0.15* (0.18÷0.66) |
0.41±0.16* (0.18÷0.76) |
0.44*±0.16 (0.18÷0.76) |
0.36±0.16 (0.18÷0.71) |
N |
0.20±0.11 (0.01÷0.53) |
0.20±0.10 (0.08÷0.34) |
0.19±0.10 (0.01÷0.34) |
0.19±0.11 (0.01÷0.32) |
0.19±0.11 (0.01÷0.34) |
0.23±0.11 (0.01÷0.34) |
0.21±0.10 (0.01÷0.34) |
0.17±0.10 (0.01÷0.32) |
0.17±0.13 (0.01÷0.32) |
0.21±0.09 (0.01÷0.34) |
0.20±0.11 (0.01÷0.34) |
0.20±0.10 (0.01÷0.34) | |
TP53 |
T |
0.10±0.06 (0.01÷0.20) |
0.08±0.05 (0.01÷0.18) |
0.09±0.06 (0.01÷0.20) |
0.11±0.06 (0.01÷0.20) |
0.10±0.06 (0.01÷0.20) |
0.10±0.08 (0.01÷0.20) |
0.10±0.06 (0.01÷0.18) |
0.10±0.04 (0.01÷0.17) |
0.10±0.06 (0.01÷0.20) |
0.10±0.06 (0.01÷0.20) |
0.10±0.07 (0.01÷0.20) |
0.10±0.05 (0.01÷0.18) |
N |
0.09±0.07 (0.01÷0.21) |
0.08±0.07 (0.01÷0.19) |
0.09±0.06 (0.01÷0.20) |
0.09±0.07 (0.01÷0.21) |
0.09±0.07 (0.01÷0.21) |
0.07±0.05 (0.01÷0.16) |
0.08±0.07 (0.01÷0.21) |
0.09±0.06 (0.01÷0.21) |
0.15±0.04 (0.09÷0.21) |
0.07±0.06 (0.01÷0.20) |
0.10±0.07 (0.01÷0.20) |
0.07±0.06 (0.01÷0.21) | |
RB1 |
T |
0.26±0.12 (0.01÷0.40) |
0.21±0.12 (0.01÷0.36) |
0.27±0.13 (0.01÷0.40) |
0.27±0.12 (0.01÷0.38) |
0.26±0.13 (0.01÷0.40) |
0.27±0.13 (0.01÷0.36) |
0.28±0.11 (0.01÷0.40) |
0.24±0.15 (0.01÷0.38) |
0.27±0.10 (0.06÷0.39) |
0.25±0.14 (0.01÷0.40) |
0.26±0.12 (0.01÷0.39) |
0.27±0.14 (0.01÷0.40) |
N |
0.25±0.11 (0.01÷0.40) |
0.26±0.11 (0.01÷0.38) |
0.26±0.11 (0.01÷0.40) |
0.23±0.10 (0.01÷0.36) |
0.26±0.10 (0.01÷0.40) |
0.23±0.14 (0.01÷0.36) |
0.26±0.10 (0.01÷0.40) |
0.23±0.12 (0.01÷0.36) |
0.22±0.10 (0.01÷0.34) |
0.24±0.12 (0.01÷0.40) |
0.25±0.10 (0.01÷0.38) |
0.26±0.12 (0.01÷0.40 | |
WIF1 |
T |
0.30±0.16* (0.08÷0.58) |
0.33±0.15 (0.14÷0.58) |
0.30±0.17 (0.08÷0.58) |
0.29±0.15* (0.09÷0.58) |
0.29±0.15* (0.08÷0.58) |
0.34±0.16* (0.09÷0.56) |
0.26±0.13 (0.08÷0.58) |
0.37±0.18* (0.08÷0.59) |
0.23±0.13* (0.09÷0.55) |
0.32±0.15 (0.09÷0.58) |
0.29*±0.16 (0.08÷0.58) |
0.32±0.16 (0.08÷0.58) |
N |
0.16±0.08 (0.01÷0.42) |
0.21±0.05 (0.15÷0.30) |
0.18±0.10 (0.01÷0.42) |
0.11±0.05 (0.01÷0.22) |
0.16±0.07 (0.01÷0.42) |
0.18±0.08 (0.08÷0.26) |
0.16±0.09 (0.01÷0.42) |
0.15±0.08 (0.01÷0.30) |
0.10±0.06 (0.01÷0.20) |
0.18±0.08 (0.01÷0.20) |
0.14±0.06 (0.01÷0.26) |
0.18±0.11 (0.01÷0.42) |

Distribution of all patients according to the years (58.68 ± 11.05).

Pre-operative contrast-enhanced CT images of three patients. A. Patient №37 - 49-year-old male diagnosed with locally advanced (T3N1M0) stage of laryngeal squamous cell non-keratinizing cancer. B. Patient №17 - 66-year-old female diagnosed with locally advanced (T3N1M0) stage squamous cell non-keratinizing cancer of mouth floor. C. Patient №41 - 72-year-old male diagnosed with locally advanced (T4aN0M0) stage laryngeal squamous cell keratinizing cancer.

The relationship between individual DNA methylation level in tumor and normal tissue in genes (CDO1, WIF1) and age in patients with different TNM classification. А. CDO1 gene, patients with T3; B. WIF1 gene, patients with T3; C. WIF1 gene, patients with T4.
Methods
Patient characteristics
The study involved 44 patients (34 men and 10 women) with HNSCC treated at A. Tsyb Medical Radiology Center, Obninsk, and P.A. Herzen Cancer Research Institute, Moscow. The study was approved by the A. Tsyb MRC Ethics Committee (approval № 634-17.11.2021).
For the first step of therapy, all patients underwent surgery. The absence of other treatments before surgery was the main inclusion criterion.
The sampling time duration was 6 months. All patients were sampled before starting further therapies. The characteristics of the patients are listed in Table 2. In total, 37 patients were diagnosed with primary HNSCC, and tumor recurrence after therapy was observed in seven cases. Squamous cell carcinoma of the tongue was observed in nine cases, cancer of the oral cavity was observed in six, cancer of the floor of the mouth was observed in three, cancer of the maxillary sinus was observed in six, and larynx cancer was observed in 20 patients. The non-keratinizing tumors were observed in 28 patients, with the keratinizing type observed in 16 patients [21]. Of the 44 patients, 26 were smokers. The mean age of the men was 58 (30÷72), and that of the women was 58 (47÷81) years old. Distribution by age in all patients is shown in Figure 1: four patients were 30–40 years of age (9%), eight patients were 41–50 years (18%), nine patients were 52–59 years (20%), 20 patients were 61–70 years (44%), and four patients were 71–81 (9%). The patients are classified according to the tumor, node, metastasis (TNM) system22, in which T - 2 (5%), T - 7 (16%), T - 20 (45%), and T - 15 (34%).
The pre-operative contrast-enhanced CT images of three patients with various diagnoses are shown in Figure 2.
Sampling
Tumor and normal tissue samples from each patient were obtained during surgery and stored at −20°C.
DNA extraction
DNA isolation from biomaterials was performed on microcolumns (K-SORB, № EX-514, Syntol, Russia) following the manufacturer's instructions.
DNA methylation analysis
Bisulfite conversion was performed using the EZ DNA Methylation-Lightning Kit (ThermoFisher EpiJET Bisulfite Conversion Kit, K1461, ThermoFisher Scientific, Waltham, MA, USA) following the manufacturer’s instructions.
Methylation of the promoter regions of the genes was performed using the methylation-sensitive high-resolution melting (MS-HRM) method using the CFX 96 Connect real-time system (BioRad, USA).
The primers for the reaction were selected using Primer Blast software (Table 3). Ready-mix (PCR-Mix, M-428, Syntol, Russia) was used for two-step PCR. The program of amplification included 95°С — 5 min; (95°С — 15 s, 60°С — 30 s, 72°С — 45 s) × 30 cycles; (95°С — 15 s, 50°С — 30 s, 72°С — 45 s) × 25 cycles23. Furthermore, the intercalating dye EVA Green (Syntol, Russia) was added to the obtained products. Each sample was run in duplicate. The melting curve was constructed according to the following program: first stage - 95° - 30 s; second stage - 60° - 10 min; third stage — melting analysis in the range of 60–90° with a 0.2° step. MS-HRM was performed using Precision Melt Analysis Software, version 3 (BioRad, USA). A CFX96 amplifier (BioRad, USA) was used for PCR and MS-HRM. The methylation level was detected by fluorescence expressed in relative fluorescence units (RFU)24.
Statistical analysis
Statistical analysis of the data was carried out using R language. The method used was a chi-squared test. P values less than 0.05 were considered statistically significant.
Results
The average methylation level results of the DNA promoter for all normal and tumor tissues of the patients and subgroups are shown in Table 4. Significant differences in DNA methylation levels between the tumor and normal tissues were found for and genes in all patients (p < 0.05). Significant differences were also observed for and genes in the tumor and normal tissues in the different subgroups (p < 0.05). The exception was the gene in a subgroup of patients with T and non-smokers. Regarding the gene, the exceptions were found in subgroups of patients with T and T, patients over 50, and non-smokers. The methylation levels of the DNA promoter of and genes were insignificant. Furthermore, no significant differences were found between the subgroups (T, T, and T stage, with primary and recurrent lesions, non-keratinizing and keratinizing SCC, before and after 50 years of age, and smokers and non-smokers) of the patients.
Afterward, significant differences were observed in the methylation level between the tumor and normal tissues only for the and genes. Using different TNM systems, we analyzed the relationship between these data and age in the patient samples (Figure 3). We observed a significant positive correlation in the methylation level in the gene with age in normal tissue from patients with T (Age/Normal: y = −0.3102 + 0.0085*x; r = 0.66; p = 0.002; r = 0.43). The correlation between the methylation level in with age in tumor tissue from patients with T was not significant but was different (Age/Tumor3: y = −0.0641 + 0.0086*x; r = 0.42; p = 0.07; r = 0.17). We observed a significant correlation between methylation level in the gene and age in the tumor tissue samples from the subgroup with T (Age/Tumor3: y = −0.4654 + 0.013*x; r = 0.59; p = 0.006; r = 0.35) and normal tissue samples in the subgroup with T (Age/Normal: y = −0.0002 + 0.0019*x; r = 0.52; p = 0.05; r = 0.27).
Discussion
Epigenetic disorders in tumor tissue cells in various types of oncopathology are as important as mutations, according to abundant literature data from the last decade. Early-stage epigenetic alterations can be identified and exploited to diagnose tumors early and predict cancer prognosis25, and DNA methylation can be used as a molecular target for cancer treatment because it is reversible via pharmacological inhibition of DNA methyltransferase.
Cysteine dioxygenase type 1 protein-coding gene and tumor suppressor. expression may be silenced by promoter DNA methylation. can augment the generation of reactive oxygen species to induce apoptosis. Many studies have shown increased promoter methylation and, consequently, suppression of the gene in various tumor cells26. In primary breast cancer patients and those with prostate cancer, the methylation level of the gene's promoter region correlates strongly with tumor development. It can be utilized as a reliable prognostic indicator27. High levels of promoter methylation have been observed in Barrett esophageal adenocarcinoma and prostate cancer patients28, 29. Our findings align with the evidence from the literature because the overall group of patients with HNSCC and subgroups had considerably higher gene promoter methylation levels in tumor tissue than in normal tissue. Moreover, significant differences were found between smokers and non-smokers.
WNT inhibitory factor 1 is a protein-coding gene that is a tumor suppressor gene. These genes are epigenetically silenced in various cancers. The protein encoded by this gene functions to inhibit WNT proteins, which are extracellular signaling molecules that play a role in embryonic development. Wnt signal transduction dysregulation can contribute to head and neck cancer. Inhibition of Wnt signaling induces apoptosis and inhibits tumor growth in many cancer types30. Mutations in the genes encoding proteins in the Wnt (Wingless/Int-1) pathway are rare in HNSCC. Thus, this pathway is not considered significant for the pathogenesis of head and neck carcinomas31.
Few studies exist on the methylation levels of the gene. Our data indicated no significant differences in normal and tumor tissues from patients in terms of the DNA methylation level of the gene.
Methylation of the gene, one of the antagonists of this pathway, is often associated with the development of this pathology32. has the highest methylation frequency in oral carcinomas. is methylated in 18% of patients with oral squamous cell carcinoma33 and 35% of patients with tongue carcinoma34, and it is significantly methylated in nasopharyngeal tumors35. In addition, non-small cell lung cancer frequently exhibits hypermethylation of the (Wnt inhibitory factor-1) promoter region, even though patient characteristics, such as age, sex, and smoking history, are unrelated to the methylation status36.
Our study showed substantial variations in the average promoter methylation levels between tumor and normal tissues in the entire patient population and in the subgroups. There were also significant differences in this parameter in the tumor tissue in the subgroups of patients with keratinizing and non-keratinizing HNSCC.
It is well known that DNA methylation is associated with cancer and aging. Aberrant DNA methylation frequently occurs with aging and illnesses such as cancer. DNA methylation status can become disrupted according to age and a particular disease stage. For example, promoter-specific hypermethylation and concurrent gene silence are linked to various malignancies37. We found a significant correlation between methylation level for the and genes in the normal and tumor tissues and age only in the subgroups with T and T. These data indicate some dependence of the methylation level on the patient's age. The individual epigenetic features of the patient’s genome likely play an essential role in the results obtained. Thus, the study should be repeated with a larger sample size, and/or patients should be stratified according to other criteria.
() is a tumor suppressor gene that inhibits cell proliferation through the Wnt/β-catenin signaling pathway. interacts with DNA repair proteins, DNA replication proteins, tubulin, and other components. It is not expressed in some types of cancer, in particular prostate, breast, and colorectal cancer. Lack of expression is associated with reduced survival in cancer patients. Moreover, regulates the response to chemotherapy in cancer cells38. The Wnt/β-catenin signaling pathway is activated by mutations in colorectal, endometrial, and prostate malignancies39. Hypermethylation of this gene has been detected in oral squamous cell carcinoma samples in some studies but not in patients with head and neck cancers. Additionally, there are no differences in the methylation of the gene between HNSCC patients and healthy individuals. The promoter was methylated in 7% of DNA samples taken from the plasma of a population free of cancer. It was unrelated to several other putative risk factors, such as age, tobacco and alcohol use, family history of cancer, diet, and nutrition. Other research failed to find a connection between clinical traits and outcomes, such as survival and aberrant methylation40, 41. Our results showed no significant differences in DNA methylation for the gene between tumor and normal tissues.
The ataxia–telangiectasia mutation ( gene product is a well-known tumor suppressor essential for maintaining genomic stability. The mutated form of the gene is involved in cell cycle control, apoptosis, oxidative stress, and telomere maintenance, and its role as a risk factor for cancer development is well established. In addition, several studies have been conducted to evaluate the risk of gene mutations for breast cancer. In addition, several studies have been conducted to evaluate the screening of gene mutations for the occurrence of breast cancer42, 43. In HNSCC, the gene promoter is a target for abnormal hypermethylation. Reduced function through epigenetic silencing is a likely mechanism contributing to HNSCC and other tumor types, given its significant role in maintaining genome integrity and the causal role that genome instability plays in cancer onset and progression. It has been found that hypermethylation of the promoter is significantly correlated with a decrease in the average survival rate of patients. Furthermore, this link is unrelated to other possible predictive elements, such as tumor size, lymph node condition, clinical stage, and history of tobacco and alcohol use. Thus, these data indicate that hypermethylation of the promoter can be used as a prognostic factor in HNSCC44. However, our data revealed no significant differences in DNA methylation levels for the gene inthe tumor and normal tissues of patients.
Functional properties caused by tumor protein p53 () gene mutations are involved in cancer development and progression45, 46. Somatic mutation is one of the most prevalent changes in the gene in human tumors. Moreover, the underlying cause of Li-Fraumeni syndrome, which predisposes patients to various early-onset cancers, is germline mutation. gene alterations are also possible prognostic and predictive indicators and prospective drug targets47. According to a thorough integrative genomic study, most somatic genomic changes in HNSCC are mutations in the gene. Invasion, metastasis, genomic instability, and cancer cell proliferation are all facilitated by mutations, which can result in a loss of wild-type activity or an increase in those activities. Interestingly, aggressiveness and worse survival following surgical treatment of HNSCC are related to disruptive mutations in tumor DNA48. Harris C.C. proposed that genetic analysis of the gene in exfoliated cells detected in either body fluids or tissue biopsies might identify people at elevated cancer risk because mutations in the gene can arise in precancerous lesions in the lung, breast, esophagus, and colon49. Hypermethylation of the promoter region of is often associated with a decrease in its activity and can even lead to its silence and, thus, to the loss of its function. This, in turn, contributes to malignant transformation50. Unfortunately, our research showed no significant differences in DNA methylation for thegene betweentumor and normal tissues in the patients.
The tumor suppressor retinoblastoma gene () is essential for controlling the cell cycle by increasing G1/S arrest and limiting growth by blocking the E2F transcription factor. Even aneuploidy can result from loss, dramatically increasing the chance of developing cancer. The gene is part of a large gene family that includes RBL1 and RBL2, each encoding the structurally related proteins pRb, p107, and p13051. One frameshift and seven non-synonymous missense mutations were discovered in (pocket domain and spacer region) sequencing analysis. In HNC tumor samples, an promoter methylation study showed that 16% of its cytosines (3% in CpG) were methylated52. Sabir M. and colleagues indicated that genetic and epigenetic changes might contribute to the pathogenesis of HNSCC in the Pakistani population53. However, our study showed no significant differences in DNA methylation forthe gene betweentumor and normal tissues in our examined patients.
Even though in our patients with HNSCC, in the tumor-associated genes , , , and , no significant changes were observed in the methylation level between normal and tumor tissue, these genes are not specific for epigenetic analysis.
We also did not find significant differences in any genes (T, T, and T stages, with primary and recurrent lesion, non-keratinizing and keratinizing SCC, age before and after 50 years, and smokers, and non-smokers) of the patients. This may be due to the small number of patients examined.
Conclusions
Thus, we can conclude that changes in the expressions of the and genes can affect the occurrence and development of HNSCC and can be considered prognostic and diagnostic markers for this pathology. Future application of molecular–genetic and epigenetic studies on HNSCC can serve as the basis for target therapy and contribute to personalized medicine.
Abbreviations
None.
Acknowledgments
None.
Author’s contributions
Kurevlev S., molecular-genetic investigation, development of research design;
Funding
The study was done with the financial support of the Russian Federation represented by the Ministry of Education and Science of Russia; Agreement dated October 7, 2021 No. 075-15-2021-1356 (internal number of the Agreement 15.SIN.21.0011); (ID: RF 0951.61321X0012).
Availability of data and materials
Data and materials used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Ethics approval and consent to participate
The study protocol was approved by the Biomedical Ethics Committee of the A.F. Tsyb Medical Radiology Research Center Branch of the Federal State Institution "National Medical Research Center of Radiology" of the Ministry of Health of the Russian Federation (protocol № 634 from 17.11.2021).
Consent for publication
Not applicable.
Competing interests
The authors declare that they have no competing interests.